Immunohistochemical Analysis of Asprosin and Meteorin-Like Peptide in Melanocytic Nevus and Malignant Melanoma
Betul Demir1, Demet Cicek1, Esma Inan Yüksel2, Nevin Kocaman3, Ilknur Calik4, Ozge Sevil Karstarli Bakay5, Yunus Gural61Firat University Hospital, Department of Dermatology, Elazig, Turkey2Biruni University Hospital, Department of Dermatology, Istanbul, Turkey
3Firat University Hospital, Department of Histology and Embryology, Elazig, Turkey
4Firat University Hospital, Department of Pathology, Elazig, Turkey
5Pamukkale University, Department of Dermatology, Denizli, Turkey
6Firat University, Faculty of Science, Department of Statistics, Elazig, Turkey.
INTRODUCTION: Objective: Asprosin (ASP) plays many important roles in the central nervous system, peripheral tissues, and organs, such as appetite, glucose metabolism, insulin resistance, and cell apoptosis. Many experimental and clinical studies have examined the effects of meteorin-like peptide (METRNL) on inflammation. Immunohistochemical studies play an important role in the diagnosis of melanocytic proliferations. This study aimed to examine the immunohistochemical analysis of ASP and METRNL in healthy skin, melanocytic nevus, and malignant melanoma and to investigate their possible role in melanocyte proliferation.
METHODS: A total of 36 skin samples, including 12 benign nevi, 8 dysplastic nevi, 6 malignant melanoma, and 10 healthy skin around lesions removed from breast tissue that was not exposed to the sun as a control group were included in this study. The skin samples were stained immunohistochemically using the avidin-biotin-peroxidase complex method.
RESULTS: ASP and METRNL expressions were detected in the epidermis and hair follicles of healthy skin. No significant difference was detected in ASP and METRNL histoscores in tumor tissues of benign nevus, dysplastic nevus, and malignant melanoma (p>0.05).
DISCUSSION AND CONCLUSION: Current findings suggest that ASP and METRNL may be associated with cell-to-cell interactions between keratinocytes, melanocytes, and inflammatory cells and may be effective in melanocyte functions. Future studies will clarify whether ASP and METRNL are potential biomarkers for melanocytic tumor development.
Manuscript Language: English