The Effect of Hypochlorous Obtained From Different Salts on Keratinocyte and Fibroblast Cells

INTRODUCTION: Hypochlorous acid (HOCl) is a physiological molecule synthesised by neutrophils during phagocytosis and plays an active role in the destruction of all microorganisms. In general, HOCl has been demonstrated to function as an antiseptic and an agent that induces apoptosis or cell necrosis in cells, depending on the ambient concentration.The aim of this study was to examine the impact of sodium hypochlorite (NaOCI) and zinc hypochlorite (ZnOCI), derived from diverse chlorine salts, on cellular proliferation and migration in keratinocyte and fibroblast cell lines in vitro.
METHODS: The effects of different concentrations of NaOCl and ZnOCl on keratinocyte and fibroblast cell lines were compared using cell viability, colony formation and wound healing assays. In addition, the presence of apoptosis in the cells was determined using the tunel method.
RESULTS: The application of NaOCI and ZnOCI resulted in a notable enhancement in colony formation within keratinocyte cell lines, whereas this effect was not observed in fibroblast cell lines. In the fibroblast cell line, the application of NaOCI and ZnOCI was observed to result in the closure of the wound site. The keratinocyte cell line did not demonstrate a comparable effect. The application of NaOCI resulted in a notable reduction in apoptosis within the keratinocyte cell line, in comparison to the control group.
DISCUSSION AND CONCLUSION: NaOCL and ZnOCl increased colony formation in the keratinocyte cell line and enhanced wound healing in the fibroblast cell line. These hypochlorous acid derivatives were not superior to each other in terms of activity on cell lines.

Keywords: NaOCI, ZnOCI, fibroblast cell line, keratinocyte cell line,