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Therapeutic Effects of Cichorium intybus L. Extract in an Experimentally Induced Insulin Resistance Model in Rats
Uğur Ermis1, Hüseyin Fatih Gül2, Sefa Gözcü3, Bengül Özdemir Sarıkaya4, Özgecan Hamdemir5, Damla Binnetoğlu11Department Of Pharmacology, Faculty Of Medicine, Kafkas University, Kars, Türkiye2Department Of Biochemistry, Faculty Of Medicine, Kafkas University, Kars, Türkiye
3Department Of Pharmacognosy, Faculty Of Pharmacy, Erzincan Binali Yıldırım University, Erzincan, Türkiye
4Department Of Histology And Embryology, Faculty Of Medicine, Kafkas University, Kars, Türkiye
5Department Of Pathology, Faculty Of Medicine, Kafkas University, Kars, Türkiye
INTRODUCTION: Insulin resistance (IR) is a central metabolic feature of polycystic ovary syndrome (PCOS). Although metformin improves insulin sensitivity, its adverse effects limit long-term use, prompting interest in herbal alternatives. The present study aimed to investigate the effects of Cichorium intybus L. ethanolic extract on PCOS-associated IR in a letrozole-induced rat model, compared with metformin.
METHODS: Twenty-four female Sprague–Dawley rats were divided into four groups: healthy control (H), PCOS-associated IR (IR), metformin-treated (MET), and Cichorium intybus ethanolic extract–treated (C.I) groups. PCOS was induced by oral letrozole administration (1 mg/kg/day) for 21 days. Following confirmation of IR by estrous cycle disruption and oral glucose tolerance test (OGTT), treatments were administered orally for 15 days. Metabolic parameters, serum insulin, glucose, lipid profile, sex hormones, and HOMA-IR index were evaluated. Skeletal muscle tissues were examined histopathologically and by immunohistochemical analysis of insulin receptor (INSR), insulin receptor subsrate 1 (IRS1), and insulin receptor subsrate 2 (IRS2) expression.
RESULTS: PCOS-associated IR rats showed impaired glucose tolerance, hyperinsulinemia, increased HOMA-IR, dyslipidemia, and hormonal imbalance. C. intybus ethanolic extract treatment significantly improved glucose tolerance, insulin levels, HOMA-IR, and lipid profile, with effects comparable to metformin. While metformin markedly restored INSR, IRS1, and IRS2 expression in skeletal muscle, C. intybus produced only moderate improvements
DISCUSSION AND CONCLUSION: C. intybus ethanolic extract improved metabolic, hormonal, and biochemical parameters in PCOS-associated IR. However, its effects on skeletal muscle insulin signaling markers were less pronounced than metformin, suggesting a complementary therapeutic role mainly through systemic metabolic regulation.
Keywords: Cichorium intybus, polycystic ovary syndrome, insulin resistance, letrozole, disease models animal.
Manuscript Language: English